Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: DJ-1 promotes necroptosis of chondrocytes by the PTEN/PI3K-AKT signaling pathway
doi: 10.1007/s00018-026-06122-3
Figure Lengend Snippet: DJ-1 Promotes TSZ + LPS-Induced Dysregulation of Chondrocyte Matrix Metabolism. ( A , B ) The protein levels of PTEN, DJ-1, Aggrecan, Collagen II, and MMP3, MMP13 were detected by western blot in human primary chondrocytes overexpressing HA-DJ-1. Corresponding total protein and β-actin were used as loading controls. ( C ) The mRNA levels of Aggrecan, Collagen II, and MMP3, MMP13 were detected by qRT-PCR in human primary chondrocytes overexpressing HA-DJ-1. ( D , E ) Immunofluorescence staining was performed to analyze Aggrecan and MMP13 expression in human primary chondrocytes overexpressing HA-DJ-1. Aggrecan (green), MMP13(red), DAPI (blue), (Scale bar: 20 μm). ( F , G ) Western blot analysis of PTEN, DJ-1, Aggrecan, Collagen II, MMP3, and MMP13 protein in different groups with pretreatment of PTEN activator Oroxin B (0.5µM, MCE). Corresponding total protein and β-actin were used as loading controls. The n values are all biological replicates. Data are presented as mean ± SD of triplicates. Statistical significance is indicated by **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05, and ns : not significant ( n = 3 each group). Data were compared using one-way analysis of variance
Article Snippet: Aggrecan (green), MMP13(red), DAPI (blue), (Scale bar: 20 μm). ( F , G ) Western blot analysis of PTEN, DJ-1, Aggrecan, Collagen II, MMP3, and MMP13 protein in different groups with pretreatment of PTEN activator Oroxin B (0.5μM, MCE).
Techniques: Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining, Expressing
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